Reintroduce Yourself to the Power of the GS FLX+ System

Don't miss the true biology -
Get more complete coverage of target regions

Perform highly accurate sequencing of long amplicons up to 800 bp on the GS FLX+ System with a simple software upgrade (Version 2.9).

  • More precise taxonomic classification of organisms in 16S and 18S rRNA samples by covering more variable regions in a single long read
  • More comprehensive detection of genetic variations in multiplex gene panels by spanning more introns and exons in a single long amplicon
  • Improved haplotyping phasing of more distantly linked variants (e.g. HLA)
  • Enhanced variant linkage in viral drug resistance studies by covering more contiguous regions of viral genomes (e.g. HIV)

Download the flyer

Example 950 bp 16S rRNA amplicon sequenced on the GS FLX+ System. The 16S rRNA genes of a mixed environmental sample were amplifed using primers spanning approximately 950 bp. The sample was sequenced using the GS FLX Titanium XL+ Sequencing Kit and processed using using v2.9 software with Flow Pattern B.

For life science research
  only, not for use in diagnostic procedures.

What’s New with GS FLX+ Software v2.9?

  • Perform highly accurate sequencing of long amplicons up to 800 bp on the GS FLX+ System with a simple software upgrade (Version 2.9).
  • Extend the range of amplicon lengths available for your targeted sequencing projects while maintaining >99% accuracy over the length of the read.
  • Sequence even longer amplicons up to 1,100 bp with custom modified pipeline settings.
  • Three available amplicon processing pipelines offer varying filtering strategies and read trimming settings for customization of sequencing results to fit project needs

GS FLX+System
Sequencing Kit XL+ XLR70
Read Length Up to 1,000 bp Up to 600 bp
Mode Read Length 700 bp 450 bp
Throughput 700 Mb 450 Mb
Reads per Run ~1,000,000
Concensus Accuracy 100.00% 100.00%
Run Time 23 hours 10 hours

Performance of the GS FLX+ System with Sequencing Kit XL+ and Sequencint Kit XLR70. Actual results depend on specific sample and genomic characteristics. *Consensus accuracy at 15x coverage E. coli.

Targeted region sequencing using GS FLX+ long amplicons. Primers A and B are incorporated at the 5' end a template-specific sequence, followed by locus-specific amplification resulting in amplicons that are ready for emPCR and sequencing. With the GS FLX+ System, hundreds of thousands of amplicons can be sequenced independently. Directionality is maintained throughout sequencing and analysis.