Discovery of novel virus in Ugandan monkey
Using the GS Junior System, a novel Simian hemorrhagic fever virus (SHFV) was discovered and characterized from a single infected wild red colobus monkey in Uganda. The virus was detected directly by sequencing RNA isolated from a blood plasma sample. De novo assembly of sequence reads revealed nearly full-length viral genome sequences of two highly divergent strains of SHFV. Read Article.
Transcriptome sequencing sheds light on the social lives of bees
To study the evolution of insect “eusociality”, a social system in which members of a female-centric colony forego their reproductive rights and devote themselves to specialized community tasks, researchers sequenced the transcriptomes of nine socially diverse bee species using the GS FLX System. Genetic differences between highly eusocial and primitively eusocial bees were associated with “signatures of accelerated evolution”. Read Article.
Whole genome sequencing of hypervirulent Chlamydia trachomatis
In an effort to determine what makes Chlamydia strains virulent, researchers performed whole genome sequencing of an unusual lymphogranuloma venereum (LGV) strain, termed L2c, which was responsible for severe hemorrhagic proctitis. Shotgun sequence reads from the GS FLX and GS Junior Systems were assembled and compared with two existing LGV strains. Analysis showed that L2c is a recombinant of invasive L2 and non-invasive D strains, revealing that recombination between strains is a mechanism for evolution of new strains with significant pathogenicity. Read Article.