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HLA

Single Pass, High-Resolution Sequencing-Based HLA Typing with GS FLX and GS Junior Systems

Perform high- and medium-resolution genotyping of the class I and II loci of the Human Leukocyte Antigen (HLA) genes using the GS GType HLA Primer Sets. 454 Sequencing Systems’ long, clonal reads provide direct, unambiguous assignment of phase for linked polymorphisms within exons. This enables third party genotyping analysis software to perform a high-resolution allele assignment without requiring additional sequencing run iterations to resolve ambiguities, as is commonly needed with traditional capillary-based Sequence Based Typing (SBT) assays.

  • Save work – Clonal sequencing eliminates the need for multiple sequencing iterations to achieve unambiguous allele identification
  • Save time – Go from sample to full genotype in as little as 3 days with the GS Junior System
  • Increase throughput – Multiplexed assay design allows up to 80 samples per sequencing run on the GS FLX System
  • Simplify analysis – Sequence output is compatible with third party genotyping software tools such as Assign™-ATF 454 from Conexio Genomics

Exons from the HLA class I and class II loci are targeted in the GS GType MR (Medium Resolution) and HR (High Resolution) Primer Sets. MR plates are used to sequence exons in column one, or can be used in combination with HR plates to sequence all exons listed. Generic DRB primers sequence multiple members of multi-gene families (i.e. DRB1,3,4,5).

High Resolution (MR + HR)
Medium Resolution (MR)  
GType MR Primer Set GType HR Primer Set
HLA-A exons 2, 3 HLA-A exon 4
HLA-B exons 2, 3 HLA-B exon 4
HLA-C exons 2, 3 HLA-C exon 4
DQB1 exon 2 DQB1 exon 3
DRB1,3,4,5 exon 2 DPB1 exon 2
    DQA1 exon 2

Table 1: MR plates are used to sequence exons in column one, or can be used in combination with HR plates to sequence all exons listed. Generic DRB primers sequence multiple members of multi-gene families (i.e. DRB1,3,4,5).

Sequence multiple samples per run

The high-throughput nature of the GS FLX and GS Junior Systems, along with multiplexed assay design, enables the sequencing of multiple samples in a single run

Samples Per Run
  Medium Resolution High Resolution
GS FLX System 80 40
GS Junior System 10 5
Product Name Pack Size Catalog Number Type
GS GType HLA MR Primer Set 1 kit (4 PCR plates with dried-down primers) 05872529001 Kit
GS GType HLA HR Primer Set 1 kit (4 PCR plates with dried-down primers) 05872537001 Kit