Products & Solutions
Amplicon Sequencing
New! GS FLX Titanium Series Amplicon Sequencing
Now bring the power of the GS FLX Titanium series to your amplicon sequencing projects. Perform high-sensitivity variant detection within a specific region of interest by generating up to 1 million reads in a single Genome Sequencer FLX instrument run. With 400 base pair read lengths, the GS FLX Titanium amplicon sequencing kits are in the range commonly used for Sanger amplicon sequencing projects. The system is ideally suited for:
- Discovery of germ-line or somatic mutations in complex samples (e.g. cancerous tumors mixed with germline DNA) based on ultra-deep sequencing.
- Analysis of viral quasispecies present within infected populations in the context of epidemiological studies (e.g. drug-resistant mutations in HIV).
- Sequencing collections of human exons from populations of individuals for the identification of rare alleles associated with disease.
- 16S rRNA sequencing for studying bacterial phylogeny and taxonomy in metagenomic analysis of mixed samples.
High Sensitivity Variation Detection: Identify rare genetic variants <1%.
Comprehensive Coverage of Target Regions: Get more complete coverage of gene loci and perform accurate SNP calling over a long single read. GS FLX Titanium reads also enable haplotyping by linking SNPs within an amplicon.
Improved Sample Throughput: Sequence hundreds of samples per run with enhanced throughput and flexible multiplexing options.
Straightforward Data Analysis: Use GUI-based Amplicon Variant Analysis software for automated variation detection and view variation of reference sequence as a graphical display.
Table 1: Targeted region sequencing using GS FLX Titanium series amplicons. Titanium primers A and B are incorporated at the 5' end of a template-specific sequence, and used in locus-specific amplification resulting in amplicons that are ready for emPCR and sequencing. With the GS FLX Titanium series, hundreds to thousands of copies of 400 bp amplicons can be sequenced independently in a single run. Directionality is maintained throughout sequencing and analysis.
Fusion Primer Ordering
Order your 454 FusionPrimers directly from Integrated DNA Technologies (IDT). Design, synthesis and purify primers for use with the 454 Genome Sequencer FLX System.