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Enabling Technology - The System

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Expanding on the proven technology and solid performance of the Genome Sequencer 20 System the Genome Sequencer FLX System is Roche's next-generation sequencing platform. Be first to the finish and benefit from:

Flexibility

Address a broad range of applications with one versatile sequencing system for the fields of:

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Speed

Generate more than 100 million bases per 7.5-hour instrument run with an average yield of greater than 400,000 reads per run.

Accuracy

Achieve single-read accuracies that are greater than 99.5% over 200 bases per read, with reads averaging 200 to 300 bases, and obtain consensus accuracies of greater than 99.99%.

Productivity

Eliminate the laborious tasks of cloning and colony picking - generate libraries of whole genomes in a single-tube.

Cost efficiency

Reduce cost per raw base with rapid, efficient amplification and sequencing through picoliter format with massive parallelization. Benefit from flexible sample-loading options that support various throughput and experimental design needs, enabling efficient use of reagents and consumables.

Ease of use

Perform data analysis without the need for enterprise scale IT solutions. A Genome Sequencer FLX run that provides 100 million bases of high quality sequence data produces only 12 - 15 gigabytes of raw bioinformatics data which can easily be analysed and stored using an conventional IT infrastructure.

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Related Info

Glossary of Terms

Resequencing

FlowMapper Software —
A software algorithm that aligns flowgrams of sequences against a reference sequence that has been converted into many ideal sub-flowgrams and built into a database.
Flowgram —
The graphic representation of nucleotide sequence for an individual read from a single well of the PicoTiter Plate device.
Flowgram Signal Space —
The quantitative representation of any base call (whether a singlet or homopolymer stretch), based on the relative signal intensity generated during an individual nucleotide “flow” (incorporation) step.
Mapped Reads —
Sequences from a Genome Sequencer 20 run that have been aligned against a reference sequence using FlowMapper software, and were found to be above the user-specified threshold.
Homopolymer —
An uninterrupted stretch of a single nucleotide.

Consensus Generation

Coverage Depth —
The total number of nucleotides from reads that are mapped to a given position.
Consensus Accuracy —
The percentage of correct calls in the Genome Sequencer 20 consensus as compared to the reference sequence.
Signal Averaging —
All aligned flow signals at each position are averaged and the final base call is done on the averaged signal.

De novo Assembly

Newbler Assembler —
A whole genome shotgun assembler using flow signals.
Nucleotide Space —
A sequence of nucleotide characters.
Q40+ —
Bases with _99.99% accuracy.
%Bases Q40+ —
The portion of an assembled genome with a quality score of 40 or higher.
N50 Contig Size —
The size of a contig above and including that half of the nucleotides covered.

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