Applications
Somatic Variation Detection
Overview
The Genome Sequencer FLX system's long reads and ability to sequence from single DNA molecules enables straightforward identification of low frequency somatic mutations. Effectively detect the spectrum of variations in specific disease-associated regions or genes by sequencing PCR products, cDNA libraries, or DNA fragments captured with NimbleGen's Sequence Capture array.
Application Note:
Publications
- Kan et al. Diverse somatic mutation patterns and pathway alterations in human cancers. (2010) Nature ePub.
- Starr et al. A transposon-based genetic screen in mice identifies genes alterated in colorectal cancer. (2009) Science 323:1747-50.
- Wheeler et al. The complete genome of an individual by massively parallel DNA sequencing. (2008) Nature 452: 872-876.
- Yeager et al. Comprehensive resequence analysis of a 136 kb region of human chromosome 8q24 associated with prostate and colon cancers. (2008) Human Genetics. ePub
Click here to see all Somatic Variation Detection publications.
Data Analysis tools:
GS Amplicon Variant Analyzer Software